Aberrant Expression of Solute Carrier Family 35 Member A2 Correlates With Tumor Progression in Breast Cancer. Phosphoserine-86-HSPB1 (pS86-HSPB1) is cytoplasmic and highly induced in rat myometrium at labour. TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult mice. Nuclear DNA was labelled with DAPI (shown in blue). Microtubule Marker (Alexa Fluor® 594), at 1/250 dilution (shown in red). Anti-Tubulin antibody YL1/2 - Loading Control. For western blot using chemiluminescent (ECL). TBX3 stimulates proliferation and stem cell self-renewal in bladder carcinoma. For western blot, milk blocking is suitable for use with fluorescent detection systems. Thymidine Kinase 2 and Mitochondrial Protein COX I in the Cerebellum of Patients with Spinocerebellar Ataxia Type 31 Caused by Penta-nucleotide Repeats (TTCCA)n. Publishing research using ab9485? Please let us know so that we can cite the reference in this datasheet.Īb9485 has been referenced in 2835 publications. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab9485 overnight at 4☌. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateĪll lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ( ab175781) secondary antibody at 1/10000 dilution Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate All lanes : Anti-GAPDH antibody - Loading Control (ab9485) at 1/2500 dilution
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